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2117 Dr Jonathan Leake University of Sheffield
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Please note: This research Programme is no longer active


 

 

Title of Research Project

The effect of mycorrhizal mycelium on the diversity, biomass and functioning of soil microbial communities and its role in carbon and nutrient cycles.

Research workers:

 

PI

Dr Jonathan R Leake

Dept. of Animal and Plant Sciences,
University of Sheffield
SHEFFIELD  S10 2TN

Tel: 0114 222 0055

j.r.leake@sheffield.ac.uk

PI

Prof David J Read

Dept. of Animal and Plant Sciences,
University of Sheffield
SHEFFIELD  S10 2TN

Tel: 0114 222 4318

d.j.read@sheffield.ac.uk

PDRA

Dr Dave Johnson

University of Sheffield,
(as above)

d.johnson@sheffield.ac.uk

Scientific activities:

The project commenced with the testing of root and fungal hyphal exclusion in-growth cores to be used for study of the effects of mycorrhizal hyphae on carbon and nutrient cycling processes. We have tested 35 m m nylon mesh (static and rotated to sever mycorrhizal hyphae), and a range of polycarbonate cyclopore membranes (0.2, 0.4 and 0.8 m m pore size) in Sourhope soil. Further development is required for the application of the cyclopore membranes due to occasional membrane failure. The rotated and unrotated nylon mesh cores were highly effective.

We have determined basal and substrate-induced respiration rates and the effect of soil moisture content on soil respiration from soil sampled from the "play area".  In collaboration with Phil Ineson’s group, a set of 35 m m pore size in-growth cores were installed in the field and the flux of 13C through hyphae linking plant roots and rhizosphere into the root-free cores was quantified (control cores were rotated to sever hyphal links immediately prior to the 13 labelling in the field).

 

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