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2138 Prof E M H Wellington University of Warwick
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Please note: This research Programme is no longer active

 


 

Title of Research Project

Assessment of chitin decomposer diversity: their rile of actinomycetes and other bacteria in C and N cycling in limed and unlimed grasslands.


Researchers

PI

Prof EMH Wellington

Department of Biological Sciences,
University of Warwick,
Coventry
CV4 7AL

Tel: 02476523184

E.M.H.Wellington@warwick.ac.uk

 

PI

Prof J Prosser

Department of Molecular and Cell Biology,
University of Aberdeen
Institute of Medical Sciences
Aberdeen, AB25 2ZD

Tel: 01224 273148

j.prosser@abdn.ac.uk

PI

Prof G W Gooday (deceased 2002)

 

 

PDRA Dr Martin Krsek Department of Biological Sciences,
University of Warwick,
Coventry
CV4 7A

Tel: 02476522431

M.Krsek@warwick.ac.uk

 

TECH's

Angela Metcalfe (now at

AstraZeneca)

Mrs L Ward

Mrs K Matthews

   

 

Abstract

A comparative study at the cell and population levels of actinomycete colonizing plant and animal litter in Sourhope grassland soils involving the molecular identification of chitanase activity. Areas of work: (a) Determine the molecular diversity of chitanase genes in soil DNA (b) Use existing gene libraries and rapid DGGE screening of rRNA extracted from soil to refine probes for identification of active mycelium (c) Analysis of active decomposers by hybridizations using permeabilised mycelium in situ in soil/litter samples and mycelium extracted in bulk (d) Use limed and unlimed plots for analysis of 14C and 15N-labelled chitin breakdown and mycelium incorporation. Establish trophic pathways and importance of hydrolytic actinomycetes. Determine the impact of environmental conditions and polluted substrates on decomposer diversity and activity.

Links

University of Warwick